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Seven wolves produce protective clothing

Shanghai Sunland Industrial Co., Ltd is the top manufacturer of Personal Protect Equipment in China, with 20 years’experience. We are the Chinese government appointed manufacturer for government power,personal protection equipment , medical instruments,construction industry, etc. All the products get the CE, ANSI and related Industry Certificates. All our safety helmets use the top-quality raw material without any recycling material.

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Solutions to meet different needs

We provide exclusive customization of the products logo, using advanced printing technology and technology, not suitable for fading, solid and firm, scratch-proof and anti-smashing, and suitable for various scenes such as construction, mining, warehouse, inspection, etc. Our goal is to satisfy your needs. Demand, do your best.

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Professional team work and production line which can make nice quality in short time.

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We abide by the privacy policy and human rights, follow the business order, do our utmost to provide you with a fair and secure trading environment, and look forward to your customers coming to cooperate with us, openly mind and trade with customers, promote common development, and work together for a win-win situation.

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Seven wolves produce protective clothing
Annexin V-FITC Kit - Apoptosis and cell viability - Kits ...
Annexin V-FITC Kit - Apoptosis and cell viability - Kits ...

The ,Annexin V,-,FITC Kit, contains reagents for the simultaneous fluorescent ,detection, of apoptotic, necrotic, and dead ,cells, through the specific binding of ,FITC,-labeled ,Annexin V, to phosphotidylserine (PS). The ,kit, consists of ,Annexin V,-,FITC,, ,Annexin V, Binding Buffer, and Propidium Iodide Solution.

Annexin V-FITC Apoptosis Kit - Abnova
Annexin V-FITC Apoptosis Kit - Abnova

1. Incubation of ,cells, with ,Annexin V,-,FITC, a. Induce ,apoptosis, by desired method. b. Collect 1-5 x 10 5 ,cells, by centrifugation. c. Resuspend ,cells, in 500 µl of 1X Binding Buffer. d. Add 5 µl of ,Annexin V,-,FITC, and 5 µl of propidium iodide (PI 50 µg/ml, optional.) e. Incubate at room temperature for 5 min in the dark.

Annexin V-FITC Apoptosis Detection Kit|Products|nacalai ...
Annexin V-FITC Apoptosis Detection Kit|Products|nacalai ...

In normal ,cells,, phosphatidylserines (PS, membrane phospholipids) are held on the inner layer of the ,cell, membrane, so ,Annexin V, does not attach to the ,cells,. During early ,apoptosis,, the PS are exposed on the outer layer, where they attach to the ,FITC,-labeled ,Annexin V, and stain the ,cell, surface green.

Annexin V-FITC / PI Apoptosis Detection Kit[211 ...
Annexin V-FITC / PI Apoptosis Detection Kit[211 ...

Introduction. Elabscience ® ,Annexin V,-,FITC,/PI ,Cell Apoptosis Detection kit, is developed to identify apoptotic and necrotic ,cells,.. ,Annexin V, is a member of the ,annexin, family, which binds to phosphatidylserine (PS) in a calcium-dependent manner. ,Annexin V,-,FITC,, the ,FITC,-conjugated format, binds specifically to the PS on the outer leaflet of apoptotic ,cell, membrane and can be detected with ...

eBioscience™ Annexin V-FITC Apoptosis Detection Kit
eBioscience™ Annexin V-FITC Apoptosis Detection Kit

During late-stage ,apoptosis,, loss of ,cell, membrane integrity allows ,Annexin V, binding to cytosolic PS, as well as ,cell, uptake of PI and 7-AAD. ,Annexin V, staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry. Host E.coli Conjugate ,FITC, Laser Blue Laser Emit 520 nm Excite 488 nm Purity > 98% pure ...

Annexin V-FITC Early Apoptosis Detection Kit
Annexin V-FITC Early Apoptosis Detection Kit

The ,Annexin V,-,FITC, conjugate is a product manufactured for ,Cell, Signaling Technology by TAU Technologies BV. Flow cytometric analysis of Jurkat ,cells, untreated (left) or treated with camptothecin (10uM, 4 hr; right) using ,Annexin V,-,FITC, Early ,Apoptosis Detection Kit,. #6592 Store at 4ºC ,Annexin V,-,FITC, Early ,Apoptosis Detection Kit, 1 ,Kit, (100 ...

Annexin V-FITC Early Apoptosis Detection Kit
Annexin V-FITC Early Apoptosis Detection Kit

The ,Annexin V,-,FITC, Early ,Apoptosis Detection Kit, enables researchers to identify early apoptotic ,cells, within a ,cell, population. ,Annexin V,-,FITC, conjugated protein binds to ,cell, surfaces expressing phosphatidylserine, an early apotosis marker. ,Cells, stained with propidium iodide (PI), a non-,cell,-permeable DNA dye, indicate necrotic ,cells,.

Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085 ...
Annexin V-FITC Apoptosis Staining / Detection Kit (ab14085 ...

Annexin V,-,FITC Apoptosis, Staining / ,Detection Kit, ab14085 is used in a 10 min, one-step staining procedure to detect ,apoptosis, by staining phosphatidylserine molecules which have translocated to the outside of the ,cell, membrane. Analysis is by flow cytometry or fluorescence microscopy.

Annexin V-FITC Apoptosis Detection Kit | PF032
Annexin V-FITC Apoptosis Detection Kit | PF032

The Calbiochem ® ,Annexin V,-,FITC Apoptosis Detection Kit, is a non-isotopic system for the identification of ,cell, membrane alterations that accompany programmed ,cell, death. ,Detection, may be performed by flow cytometry or by fluorescence microscopy.

Annexin V-FITC Apoptosis Detection Kit|Products|nacalai ...
Annexin V-FITC Apoptosis Detection Kit|Products|nacalai ...

In normal ,cells,, phosphatidylserines (PS, membrane phospholipids) are held on the inner layer of the ,cell, membrane, so ,Annexin V, does not attach to the ,cells,. During early ,apoptosis,, the PS are exposed on the outer layer, where they attach to the ,FITC,-labeled ,Annexin V, and stain the ,cell, surface green.

Annexin V - FITC Apoptosis Detection Kit - Leinco Technologies
Annexin V - FITC Apoptosis Detection Kit - Leinco Technologies

Annexin V,, a 35-36 kDa phospholipid-binding protein, has a high affinity for PS (KDa=7nmol/l). Once ,Annexin V, is bound to the apoptotic ,cell, it acts as a beacon for ,cells, that have undergone ,apoptosis,. ,Annexin V, is often conjugated to ,FITC, so the amount of signal can be measured and analyzed from labeled ,cells, using a flow cytometer.

Annexin V-FITC Apoptosis Detection Kit (APOAF) - Technical ...
Annexin V-FITC Apoptosis Detection Kit (APOAF) - Technical ...

The ,Annexin V,-,FITC Apoptosis Detection kit, detects apoptotic ,cells, by flow cytometry. The annexins are a group of homologous proteins which bind phospholipids in the presence of calcium.1,2 ,Annexin V,-,FITC, is a fluorescent probe which binds to phosphatidylserine in the presence of calcium. ,Apoptosis,, or programmed ,cell

eBioscience™ Annexin V-FITC Apoptosis Detection Kit
eBioscience™ Annexin V-FITC Apoptosis Detection Kit

During late-stage ,apoptosis,, loss of ,cell, membrane integrity allows ,Annexin V, binding to cytosolic PS, as well as ,cell, uptake of PI and 7-AAD. ,Annexin V, staining, paired with 7-AAD or PI is widely used to identify apoptotic stages by flow cytometry. Host E.coli Conjugate ,FITC, Laser Blue Laser Emit 520 nm Excite 488 nm Purity > 98% pure ...

Annexin V Detection Kits | PromoCell
Annexin V Detection Kits | PromoCell

Annexin V detection kits, and individual reagents allow to identify early apoptotic ,cells, due to our highly sensitive probes. In combination with flow cytometry and fluorescence microscopy, the stained ,cells, reveal early ,cell, surface changes.

Annexin V-FITC Kit - Apoptosis and cell viability - Kits ...
Annexin V-FITC Kit - Apoptosis and cell viability - Kits ...

The ,Annexin V,-,FITC Kit, contains reagents for the simultaneous fluorescent ,detection, of apoptotic, necrotic, and dead ,cells, through the specific binding of ,FITC,-labeled ,Annexin V, to phosphotidylserine (PS). The ,kit, consists of ,Annexin V,-,FITC,, ,Annexin V, Binding Buffer, and Propidium Iodide Solution.

FITC Annexin V Apoptosis Detection Kit with PI
FITC Annexin V Apoptosis Detection Kit with PI

FITC Annexin V Apoptosis Detection Kit, with PI - BioLegend's ,FITC Annexin V Apoptosis Detection Kit, with PI has been specifically designed for the identification of apoptotic and necrotic ,cells,.,Annexin V, (or ,Annexin, A5) is a member of the ,annexin, family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner.

Annexin V-FITC Apoptosis Detection Kit
Annexin V-FITC Apoptosis Detection Kit

In normal ,cells,, phosphatidylserines (PS, membrane phospholipids) are held on the inner layer of the ,cell, membrane, so ,Annexin V, does not attach to the ,cells,. During early ,apoptosis,, the PS are exposed on the outer layer, where they attach to the ,FITC,-labeled ,Annexin V, and stain the ,cell, surface green.

Annexin V-FITC Apoptosis Detection Kit (50 assay)
Annexin V-FITC Apoptosis Detection Kit (50 assay)

Annexin V, has a high affinity in a Ca2+-dependent manner to negatively charged phospholipid phosphatidylserine, which is found on the outer ,cell, membrane early during ,apoptosis,. The ,Annexin V,-,FITC kit, employs ,FITC, conjugated ,Annexin V, in concert with propidium iodide (PI). As the ,cell, membrane becomes increasingly permeable during the later ...

Annexin V-FITC Apoptosis detection Kit
Annexin V-FITC Apoptosis detection Kit

Annexin V,-,FITC Apoptosis detection Kit, PRODUCT INFORMATION & MANUAL 5 8 Preparation of Reagents Dilute Binding Buffer (4x) 1:4 in distilled water (50 ml binding buffer and 150 ml distilled water). 9 Test Protocol 1. Wash ,cells, in PBS by gentle shaking or pipetting up and down. 2. Resuspend ,cells, in 200 µl Binding Buffer (1x); ,cell, density should be 2-5x105/ml.